福岡県農業総合試験場研究報告

ヒラナス（Solanum　integrifolium）細胞選抜系統の青枯病抵抗性

［摘要］ナス青枯病菌（Ralstonia　solanacearum）の培養ろ液および菌体成分に対して抵抗性を示したヒラナス（Solanum　integrifolium）のプロトプラストより再生した系統のナス青枯病抵抗性を評価した。ヒラナス実生に比較して最も発病度が低かった細胞選抜系統は、ナス青枯病菌の培養ろ液で選抜したものであった。また，組織内の青枯病菌密度も低い傾向にあった。しかし、これらの系統の発病度の差は、発生初期に大きいものの、その後次第に小さくなった。これらのことから、培養ろ液による細胞選抜は、萎凋抵抗性と菌の増殖抑制能を併せ持つ発病遅延型の抵抗性を示す系統の獲得に有効と推察される。また、培養ろ液で選抜した系統の自殖第一代を台木として用いた接ぎ木ナスでも高い青枯病抵抗性を示す系統が得られた。

［キーワード：ヒラナス、細胞選抜、青枯病抵抗性、発病遅延］

　The Bacterial Wilt-Resistibility of a Regenegated Strain of Solanum integrifolium Obtained Through In Vitro Cell Selection. HIRASHIMA Keita, Masaaki KOGA and Takao NAKAHARA(Fukuoka Aguricultural Research Center, Chikushino, Fukuoka 818-8549, Japan)Bull. Fukuoka Agric. Res. Cent.19: 55 - 59 (2000)

　An examination was made of the bacterial wilt resistibility of a strain of Solanum integrifolium , that was regenerated from protoplasts of those S. integrifolium　which resisted both the culture filtrate and bacterial components of Ralstonia solanacearum. In comparison with S. integrifolium seedling plants, the lowest disease severity was reached by plants of that strain which was subjected to in vitro cell selection with the culture filtrate of R. solanacearum. Also, the density of R. solanacearum in their tissue tended to be lower. However, the differences in disease severity between such conditioned plants were significant at the initial time of wilting occurrence and then gradually decreased. These findings lead to a thought that the in vitro cell selection by means of the culture filtrate could produce some effect on obtaining strains with a pathogeny delayed type resistibility, that may be exhibited on the bases of the wilt resistibility as well as anti-proliferation ability against some bacteria.