　ナス台木における青枯病抵抗性等の優良品種の育種期間を短縮するため，ヒラナスの小胞子を培養して植物体を再生させる技術を確立した。効率的にカルス形成を誘導するには， 4～ 7 mm程度の花蕾から小胞子を単離し，滅菌水中で35℃， 4日間処理して，多量要素を 1/2量とし，ショ糖20 g/l，NAA 0.5 mg/lおよびBA 0.5 mg/lを添加したNitsch & Nitsch培地に，小胞子の密度 5×10⁵/mlで行う培養条件が適していた。形成されたカルスを，ショ糖20 g/l，IAA 0.2 mg/l，Zeatin 4 mg/lおよび寒天 8 g/lを添加したMS培地で培養するとシュートが再生し，フロリアライトを利用して発根させることで，倍加半数体を含む多数の再生植物が得られた。

　Plant regeneration in Solanum integrifolium microspores culture. TAKATA Kinuko, Yumi SAIKI, Keita HIRASHIMA and Takao NAKAHARA (Fukuoka Agricultural Research Center, Chikushino, Fukuoka 8188549, Japan) Bull. Fukuoka Agric. Res. Cent. 25:2328　(2006)

　In order to shorten the breeding term of eggplant rootstock, we established the technique to regenerate through callus from microspores in Solanum integrifolium. For the efficient callus induction, microspores were isolated from 4-7 mm length of flower buds and prepared in sterilized water at 35℃ for 4 days before the microspore culture. After the treatment, calli were induced from the microspores in Nitsch & Nitsch medium with half strength of macronutrient, 20g/l sucrose, 0.5 ml/l NAA and 0.5 mg/l BA at a microspore density of 1×10⁵ /ml. For the shoot formation, the calli were transferred to MS medium containing 20g/l sucrose, 0.2 ml/l IAA, 4 mg/l zeatin and 8g/l agar. Subsequently, rooting from the shoots facilitated in Florialite. As a result, many regenerated plants containing doubled haploids were obtained.　