福岡県農業総合試験場研究報告22(2003) pp 99 - 102
逆転写遺伝子増幅法による温州萎縮ウイルスの高感度検出
下村克己・野口保弘
（果樹苗木分場）
温州萎縮ウイルスの高感度検出にRT-PCRおよびIC-RT-PCR法が適用可能かどうか検討した。その結果，新たに設計したプライマー [CP-L1(+)，CP-R(-)]を用いたRT-PCR法は，今回供試したSDVの2系統およびその近縁ウイルスであるCiMVの3系統に有効であった。IC-RT-PCR法の検出感度は， ELISA法の30倍以上であった。また，ELISA法では検定が不可能な冬季のカンキツの葉や樹皮からもRT-PCR法によってSDVの検出が可能であった。
以上のことから，RT-PCRおよびIC-RT-PCR法は，SDVおよびCiMVの高感度検出に有効であると考えられた。
[キ−ワ−ド：カンキツ，温州萎縮ウイルス，RT-PCR，IC-RT-PCR]
Sensitive Detection of Satsuma Dwarf Virus by Reverse Transcription Polymerase Chain Reaction. SHIMOMURA Katsumi and Yasuhiro NOGUCHI (Fukuoka Agric. Res. Cent., Chikushino, Fukuoka 818-8549, Japan) Bull. Fukuoka Agric. Res. Cent. 22: 99 - 102 (2003)
We examined whether reverse transcription polymerase chain reaction (RT-PCR) and immunocapture RT-PCR (IC-RT-PCR) methods were suitable for sensitive detection of satsuma dwarf virus(SDV) in citrus. All tested strains of SDV as well as the closely related virus, citrus mosaic virus, were detectable only by RT-PCR using a new primer pair, [CP-L1(+), CPL-R(-)], designed based on the nucleotide sequence of coat protein region of SDV-58. The detection ability of IC-RT-PCR with use of the new primer pair was 30 times higher than that of enzyme-linked immunosorbent assay. RT-PCR was useful for detection of SDV in citrus leaves and bark in winter. These results indicated that RT-PCR and IC-RT-PCR methods were useful for sensitive detection of SDV in citrus.
[Key words: citrus, satsuma dwarf virus, sensitive detection, RT-PCR, IC-RT-PCR]
