福岡県農業総合試験場研究報告14（1995） pp 87 - 91
フキ組織培養苗の増殖と馴化
古賀正明・平島敬太・中原隆夫
フキの茎頂由来の組織培養苗について，腋芽増殖のための好適培養条件，増殖率並びに馴化法について検討し，以下の事項を明らかにした。@フキの茎頂由来の組織培養苗を２ｍｇ／ＬのＢＡを添加した１／２ＭＳ培地に移植し，生じた腋芽を分割し０．０１ｍｇ／ＬのＮＡＡを添加した１／２ＭＳ培地（発根培地）に移植して培養することにより，１００日間で約４０倍に増殖することができる。A増殖したシュートを発根培地で再度１６日間培養後，バーミキュライトを入れた９８セルのセル成型トレイに移植し，２週間ポリ塩化ビニリデンフィルムで被覆することにより，高い生存率かつ良好な生育状態で馴化できる。B上記方法により，増殖開始後約１３４日で定植用苗の供給が可能である。
［キーワード：フキ，組織培養，増殖，馴化］
Micropropagation and Acclimatization of Tissue Culture Plantlets of Japanese Butterbur (Petal japonics Miq. ). KOGA Masaaki, Keita HIRASHIMA and Takao NAKAHARA. (Fukuoka Agric. Res. Cent. , Chikushino, Fukuoka 818, Japan) Bull. Fukuoka Agric. Res. Cent. 14 : 87-91 (1995)
In order to develop the Supply technique of tissue culture p1antlets of Japanese Butterbur (Petal japonics Miq. ) , conditions for micropropagation by means of axillary bud and tTor acclimatization were optimized and propagation rate were elucidated. Tissue culture plantlets are able to be propagated about fourtyfTold by 100 days through transplanting the axillary buds generated from the plantlets on the 1/2MS solid media supplemented with 2mg/L BA to the same media containing 0.01mgA NAA (root inducing medium). The plantlets propagated through above method and followed by 16 days culture on the root inducing media are able to be acclimatized with high viability and quite healthy by transplanting the cells in a 98 wells-tray filled with vermiculite and wrapped tTor 2 weeks. By these methods, the trasnsplantable plantlets to soil are ready for supplying by 134 days since propagation starts.
[Key words : Japanese butterbur (Petal japonicus Miq.), Tissue culture, Micropropagation, Acclimatization]
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